Probiotics for Aquaculture - Use of Thiobacillus ferrooxides

How much quantity of T ferrooxidans to be used ?
Use at the level of about 100 g. of 6000 Million CFU/g. once in 15 days in P. monodon culture with stocking densities of about 100,000 to 300,000 / Ha. 

In Intensive System, with stocking densities of about 300,000 to 500,000 / Ha use @ 200 g.of 6000 M CFU/gm once in 10 Days.

In Super Intensive System, with stocking densities of more than 500,000 / Ha , use @ 125 .g of 6000 M CFU / g. every alternate day. 

References :
The ferrous iron oxidation kinetics of Thiobacillus ferrooxidans in batch cultures
 
Applied Microbiology and Biotechnology 
Publisher : Springer-Verlag GmbH 
ISSN : 0175-7598 (Paper) 1432-0614 (Online) 
DOI : 10.1007/s002530051467 
Issue : Volume 51, Number 6
Date : June 1999 
Pages : 813 - 819
 

M. Boon A1, C. Ras A1, J. J. Heijnen A1 
A1 Department of Biochemical Engineering, Kluyver Laboratory of Biotechnology, Julianalaan 67, 2628 BC Delft, The Netherlands e-mail: M.Boon@stm.tudelft.nl Tel.: +31-15-2781551 Fax: +31-15-2782355

Paul, E.A., Clark, F.E. 1996. Soil Microbiology and Biochemistry. Academic Press. San Diego, CA. 340 pp.
Chapelle, Francis H. 1993. Ground-Water Microbiology and Geochemistry. John Wiley & Sons, Inc. New York, NY. Pp 98-99.

Atlas, Ronald M., Parks, Lawrence C. 1993. Handbook of Microbiological Media. CRC Press. Boca Raton, FL. 1079 pp.

Holt, J.G., N.R. Krieg, P.H.A. Sneath, J.T. Staley, and S.T. Williams. 1994. Bergy’s Manual of Determinative Microbiology. Ninth Ed. Williams and Wilkins. Baltimore, MD. 787 pp.

Optimal conditions for bio-oxidation of ferrous ions to ferric ions using Thiobacillus ferrooxidans Bioresource Technology, Volume 85, Issue 3, December 2002, Pages 225-234 
S. Malhotra, A. S. Tankhiwale, A. S. Rajvaidya and R. A. Pandey

American Society of Microbiology: 
http://www.asm.usa.org/ 

Institute of Microbial Ecology:

http://commtechlab.msu.edu/sites/dlc-me/ 

Copyright © 2006 Som Phytopharma India Ltd. All Rights Reserved.

a dristi design