Ticknil (Tick and tsetse fly in pet animals)

TICKNIL
Tick and Tsetse Fly in Pet Animals

INTRODUCTION

Worldwide cost for both the control of tick and tick borne diseases and losses is estimated at US $ 1318 per head of Cattle at 1996.
(de Castro, 1997).

A review by Norval (1990) on the effects of Amblyomma hebraeum and Rhipicephalus appendiculatus on meat and milk production in Zimbabwe using computer simulation. Models indicated that the reduction of live weight gain caused by a feeding session of each adult female tick was approximately 10g for A. hebraeum and 4 g for R. appendiculatus.

Effective strategies for exclusion or eradication of livestock pests must be based upon detailed knowledge of the pest's biology, host preference, and susceptibility to pesticides. In addition, those factors that limit the pest's distribution and methodologies for its surveillance must also be known

Important tick-borne diseases of human and livestock in India
Tick-borne diseases (TBD) Pathogen/Parasite Tick vector Host  Vaccine status
against TBD
Kyasanur Forest Disease (KFD) KFD virus Haemophysalis spinigera Man Chick embryo tissue culture vaccinea
Crimean Congo Hemorrhagic Fever (CCHF) CCHF virus Hyalomma spp? Man Nil 
Indian Tick Typhus (ITT) Rickettsia conorii Rhipicephalus sanguineus Man Nil 
Bovine Tropical Theileriosis Theileria annulata anatolicum Hyalomma anatolicum  Cattle Attenuated macroschizont 
vaccineb
Babesiosis  Babesia bigemina  Boophilus microplus  Cattle Nil
  B. motasi Haemaphysalis sp Goat Nil
  B. canis R. sanguineus Dog Nil
  B. ovis Rhipicephalus sp Sheep Nil
  B. equi Hyalomma sp Horse Nil
Anaplasmosis Anaplasma B. microplus Cattle  
  marginale  - Bufallo  
    - Sheep  Nil
Ehrlichiosis  Ehrlichia bovis Hyalomma sp  Cattle  Nil
  E. canis  R. sanguineus  Dog  Nil
(S. Ghosh, P. Azhahianambi & M.P. Yadav; Upcoming and future strategies of tick control: a review; J VECT BORNE DIS 44, JUNE 2007)

As a whole, ticks are the most versatile vectors, for they parasitize all vertebrate groups except fish. The tick-borne diseases that they transmit are among the most significant animal health deterrents to efficient livestock production. The methods of pathogen transmission employed by ticks are both mechanical and biological. In the case of soft ticks belonging to the family Argasidae, the ability of some individuals to survive for 3 years or more between blood meals permits them to assume the dual role of vector and reservoir, which is particularly important in the transmission of African swine fever virus.

Argas persicus is an external parasite of poultry and wild birds, and is also found on mammals. It is more common in warm climates, spends little time on host, and may also transmit Spirochaetosis and Pasteurella infection. These parasites are more likely to be a problem of small scale poultry production in the tropics, than in commercial poultry in temperate climates. 

Dermacentor variabilis is a dog tick found throughout the United States. The American dog tick is a three-host tick - each development stage feeds on a different host.

The female feeds for 7 to 10 days, drops to the ground, and after several days lays 4,000 - 6,500 ellipsoidal eggs over a 14 - 32 day period and then dies. It may transmit Rocky Mountain spotted fever and Tularemia (hunter's disease).

Rhipicephalus sanguineus (The Brown dog tick or kennel tick) is exclusively a dog tick and one of the most widely distributed tick on the world. It is a putative vector of Ehrlichia canis, a blood protozoan parasite that causes canine Ehrlichiosis. Other pathogens are Rickettsia rickettsia, R. belli, R. rhipicephali, R. montana and Babesia canis, another protozoan parasite that causes canine Babesiosis. 

Rhipicephalus appendiculatus has been incriminated in the transmission of bovine babesiosis, other pathogens of the East Coast fever syndrome, louping ill, Nairobi sheep disease, and Kisenly sheep disease.

Ixodes ricinus, the European castor bean tick, is responsible for transmitting the causative agents of bovine babesiosis, bovine anaplasmosis, louping ill, and tick-borne fever of cattle, sheep, and goats. 

Deer tick transmits Lyme disease with symptoms like Rash, fatigue, muscle and joint pain 

Flea and lice may transmit Plague typhus which shows Fever, skin rashes 

Wood tick transmits the Rocky Mountain spotted fever where Chills, fever, rash, leg pain will be common symptoms. 

The louse fly, Hippobosca longipennis), which inflicts a painful bite, is an ectoparasite of all hairy animals, including livestock, dogs, cats, and wild game.

The southern cattle tick, Boophilus microplus, is a vector of bovine babesiosis, bovine anaplasmosis, and benign bovine theileriosis. This tick is found is the hotter and more humid parts of the world. B. annulatus, the cattle tick is a closely related species.

Amblyomma variegatum, the tropical bont tick is a common vector of Cowdria ruminantium, which is the etiological agent of heartwater that affects cattle, sheep and goats. A. hebraeum, the bont tick, is a common vector of heartwater 

Tsetse fly causes Sleeping sickness and attacks nervous system resulting in a prolonged sleep. Although tsetse flies are limited in their distribution to sub-Saharan Africa, the importance of the animal trypanosomiases (nagana of cattle) on that continent ranks tsetse as one of the world's major arthropod-vector groups. The very complex developmental cycle of the trypanosome within the tsetse vector is further complicated by several of other factors related to the biology of the vector, pathogen, and host. Not only are the various species of tsetse flies characterized by differences in their distribution, biology, and host preferences, but even within the same species environmental factors (especially humidity, temperature, and vegetation), densities and composition of mammalian hosts, and vector population densities affect their epidemiological role. In addition, there are wide intraspecific variations in both morphology and pathogenicity of trypanosomes. Certain parasite antigens that stimulate production of protective antibodies by the host change before the parasites are completely eliminated; new antibodies are then produced by the host, and the parasites change their antigenic constitution again to maintain themselves. 

The sheep scab mite, Psoroptes ovis

The New World screwworm, Cochliomyia hominivorax

Licking fly, Musca vitripennis are also important parasites on animals 

Ceratopogonidae (biting midges), Simuliidae (black flies), Oestridae (bot flies), Chloropidae (eye gnats), Sarcophagidae (flesh flies), Ixodidae (hard ticks), Tabanidae (horse flies and deer flies), Culicidae (mosquitoes), Muscidae (muscoid flies), and Cuterebridae (robust bot flies) are some other ectoparasites affecting animals.

Common Signs
Anemia. 
Skin blemishes. 
Occasionally paralysis from toxins in the tick saliva. 
Emaciation. 
Weakness. 
Reduced productivity. 

Diagnosis

Identification of the presence of the parasites.

PREVENTION

Elimination of cracks and crevices in the animal housing. 

CONVENTIONAL CURATIVE PRACTICES 
1

Permethrin dips are a good tool for use on dogs but there is something about Permethrin that just does not get along with cats.

2 Delltamethrin Dips
3

Preventic Tick Collars are popular with people whose dogs are allowed to roam areas where ticks are common. These areas include hunting preserves, public areas where dogs are allowed on leash and even areas you would not suspect: grooming parlors and veterinarian offices. 

4 Pyrethrin Pet Spray used on a daily or as needed basis. 
5 Organophosphate Pour On.
6 Baits and Traps
7

Shampoo compositions comprising a detergent , juvenile hormone-like nitrogen containing heterocyclic compounds,insect growth regulators such as methoprene or fenoxycarb, synthetic pyrethroids thereof, wherein the dose of the active compound is sufficient for leaving on the haircoat of a warm-blooded animal after rinse out the shampoo an ovicidally and/or insecticidally and/or acaricidally residual effective amount against ectoparasites of said compound. 
(Abstract of EP0714601)

8

Recently a vaccine against Boophilus microphus was developed in Cuba (Boue et al., 1998); but its efficacy against other species of ticks has not been demonstrated.

All the above practices are not only non sustainable, but also endangers biological diversity and environmental quality. 

Therefore the need arises for an alternate environment friendly program.

TICKNIL
Tick control may be attempted by Chemotherapeutic, Immunological, Herbal, Mineral and Microbiological approaches. 
CONTENTS 
B. Bassiana
M. Anisopliae
Alternative to Ivermectin
Oxy Tetracycline
Proflavine
Anonaine 
Neem Leaf Extract
Pepaver
Brewer's yeast
Garlic Extract
Cobalt
Zinc
MODE OF ACTION
ODOUR

Taken orally, TICKNIL produces an odor that is undetectable by humans and is loathed by fleas and ticks, mosquitoes, and black flies. 
Taken regularly, TICKNIL makes the animal less attractive to infestation. 

MICROBIOLOGICAL

Biological control using entomopathogens (bacteria, fungi, viruses, nematodes or protozoa) is one of the alternatives in reversing agriculture's hazardous dependence on synthetic pesticides and establishing a more environmentally friendly paradigm. 
Mwangi et al. (1994) screened isolates of B. Basiana and M. Anisopliae against Rhipicephalus appendiculatus and observed high mortality than unfed adults. Both fungal species induced reduction in engorgement weights, fecundity and egg hatchability in adult A. variegatum and Rhipicephalus appendiculatus (Kaaya et al., 1996)

CHEMOTHERAPEUTIC APPROACH: 

Recent field reports of ivermectin resistance amongst ticks necessitates the search for another systemic antibiotic for the control of Ticks. 
Hence in TICKNIL an alternative is used in the place of Ivermectin.

Sterility was observed in healthy tsetse flies fed with tetracycline (2500 μg/ml) due to damage to themycetome bacterial endosymbionts.
Closantel @ 5 mg/ Kg 

HERBAL APPROACH
Alcoholic extracts of sitaphal (Annona squamosa) and Neem (Azadirachta indica) are being evaluated for their acaricidal property against different life stages of H. a. anatolicum and B. microplus. 

(S. Ghosh, P. Azhahianambi & M.P. Yadav; Upcoming and future strategies of tick control: a review; J VECT BORNE DIS 44, JUNE 2007)

MINERAL APPROACH
Cobalt
Zinc
TOXICOLOGY
B. Bassiana

The acute oral toxicity/pathogenicity of the technical grade active ingredient (TGAI) in the rat was determined following a single exposure to 10^8 colony forming units (CFU). The organism was not infectious to the rat and total clearance from the animal was projected to occur in 23 days. In a single 24 hour dermal exposure of two grams of the technical powder, erythema and edema were observed in 30% of the animals. These symptoms cleared in 1- 7 days and indicated that the material was a moderate dermal irritant. These results were classified as Toxicity Category IV. A bovine corneal opacity and permeability assay was conducted to project the potential irritancy of the technical powder. The results indicated that the technical powder might be a slight irritant. The results of a primary eye irritation study confirmed that the technical powder was a slight irritant to the eye (Toxicity Category III). A study was conducted in the rat in which the animals were exposed by intraperitoneal injection to 10^7 CFU of the material (MRID 43294201). The animals were unaffected by the test material during the 29 day observation period. In a rat intratracheal toxicity study, rats were exposed to 10^7 CFU per animal. There were small tan nodules in the lungs of the test animals following exposure. These lesions reversed as the study progressed and the author opined that they would be totally reversible if more time were allowed. Total clearance of Beauveria bassiana occurred within 15 days. There were no effects on survival and the test material was not found in any tissue outside the lungs. The organism was not considered toxic or pathogenic in this test animal. Instability of the conidia of Beauveria bassiana outside its natural environment, the soil is well documented. Residues should not be present because spores are not viable 96 hours following application.
(http://pmep.cce.cornell.edu/profiles/biopest-biocont/bioinsect/beauv_bass/bbass_pet_0698.html)

M. Anisopliae

A single dose of 2.2 x 10^8 CFUby oral route and 1.1 x 10^8 CFU by pulmonary administration givento Sprague Dawley rats. Clearance was estimated by means of faeces collection (Oral assay) and lungs examination (Pulmonary assay). Gross necropsy was performed on all animals at interim and final sacrifice. There were no deaths, no significant infection of test animals and no evidency of pathogenicity or treatment related toxicity. It was concluded that, at the tested doses, Metarhizium anisopliae strain LMA-06 is not pathogenic following oral or pulmonary administration.
(http://www.scialert.net/qredirect.php?doi=pjbs.2005.969.973&linkid=pdf) 

Proflavine

Is a flavine derivative. Bactericide. Inhibits in vitro s. aureus, pneumococcus etc.Widely used in general surgery, opthalmology etc. Non toxic at 1 ppm level.

LEVEL OF INCLUSION
Level of Inclusion Suggested: 1-2g/ Kg Feed once in 60 Days
REFERENCES: 
1

ANONYMOUS. 1987. Pests of plants and animals: Their introduction and spread. CAST Report No. 112:1-40

2

MORGAN, N. O. 1988. Potential Impact of Alien Arthropod Pests and Vectors of Animal Diseases on the U.S. Livestock Industry, In CRC Handbook of Pest and Management in Agriculture, Vol. I. Boca Raton, FL:CRC Press, pp. 99-105.

3

STRICKLAND, R. K., GERRISH, R. R., HOURRIGAN, J. L., and SCHUBERT, G. O. 1976. Ticks of veterinary importance. USDA Agri. Handbook No. 485:1-122.

4

Jongejan F, Uilenberg G. The global importance of ticks. Parasitology 2004; 129: S1–S12.

5

Ghosh S, Azhahianambi P, de la Fuente J. Control of ticks of ruminants, with special emphasis on livestock farming system in India—present and future possibilities for integrated control: A review. Exp Appl Acarol 2006; 40: 49–66.

6

Martins JR, Furlong J. Avermectin resistance of the cattle tick Boophilus microplus in Brazil. Vet Record 2001; 149: 64.

7

Choudhry RT, Vasanthi C, Latha BR, John L. In vitro effect of Nicotiana tabacum aqueous extract on Rhipichepalus haemophysaloides ticks. Indian J Anim Sci 2004; 74: 730–1.

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