METHOD OF ANALYSIS OF STP-24
Following tests may be conducted before and after treatment.
After treatment means after 72 Hours of applying STP-24.
TEST 01 - Odour
Deodorization of faecal matter by degradation can be tested by spraying about 250 ml/ 10 sq. m before and after treatment on an open area
Deodorized effect can be observed and it will be found that odor is barely sensible.
That is STEP-24 has decomposed odorous substances very strongly from the offensive
Target.
TEST 02 - Biological Performance
Cultivation test of red chillies using the treated waste water may be conducted.
Untreated After Treatement
50X 100x 150 x 50X 100x 150 x
Height of plants (cm)
Yield per plant (Dry Chillies with 20% moisture)
Results indicate that the treatment improves significantly height of plants and yield of chillies compared to untreated plots..
TEST 03 - H2S
| Sr. No. |
Test |
Observation |
Inference |
| 1 |
Odour |
Distinct odour of rotten eggs
|
H2S present
|
| 2 |
Lead acetate test:
Dip a strip of paper in lead acetate solution and allow it to dry partially till the strip is moist (do not completely dry).
Take 0.2g sample in a dry test tube and heat till vapours arise. Hold the moist lead acetate paper at the mouth of the test
tube |
Black precipitate or colouration on the paper
|
H2S present
|
Test for the above before and after treatment.
TEST 04- Indole
Preparation of Kovac’s reagent:
1. Take a 250ml beaker with 75ml of amyl alcohol.
2. Add 25ml conc. HCl to the amyl alcohol.
3. Dissolve 5g of N, N – Dimethyl – 4 – Amino Benzaldehyde in the above solution.
4. Transfer into a dark brown glass bottle and store at 4oC.
| Sr. No. |
Test |
Observation |
Inference |
| 1 |
Kovac’s reagent test:
Take 0.2g sample in a test tube + 4ml double distilled universal solvent. Vortex the sample thoroughly. Centrifuge the sample at 16,000 rpm for 30 minutes at 4 degrees C..
Take the supernatant liquid in a fresh test tube and add a few drops of Kovac’s reagent along the sides of the test tube. Let the test tube stand for 15 minutes.
|
A pinkish-red ring is seen at the junction of the two liquids
|
Indole present
|
Test for Indole before and after the treatment.
TEST 05 - Ammonia:
1. Take 0.2g of sample + 2ml of 0.2N NaOH + Heat the test tube till vapours form.
2. Hold a wet red litmus paper at the mouth of the test tube
> litmus colour changes to blue
> ammonia present
3. Take a glass rod dipped in conc HCl and hold it at the mouth of the heated test tube containing the sample
> dense white fumes of NH4Cl formed
> ammonia present
| Sr. No. |
Test |
Observation |
Inference |
| 1 |
Litmus Test
0.2g sample + 2ml 0.2N NaOH. Heat the test tube till vapours arise. Hold a moist red litmus paper at the mouth of the test tube. |
Litmus paper changes colour from red to blue
|
Ammonia present
|
| 2 |
HCl Test
Take 0.2g sample in a test tube and heat. Hold a glass rod dipped in conc. HCl at the mouth of the test tube. |
Dense white fumes of NH4Cl formed
|
Ammonia present
|
Test for Ammonia before and after the treatment.
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Dr
Venkatesh Devanur, Director R & D, RADOSOM, a Govt of India, Dept of Scientific and Industrial Research recognized R & D Centre has recognized the situation you are in and would like to offer professional advise in this regard.
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